Medical Products Supplies Companies

Aztec House 397-405 Archway Road, N6 4ER London
United Kingdom of Great Britain and Northern Ireland
Telephone +44 203 930 8101
info@pcrbio.com
http://www.pcrbio.com

Product Categories

Product categories

03 Diagnostic Tests
03.06 Genetic testing, molecular diagnostics
03.06.03 Polymerase chain reaction (PCR)

Polymerase chain reaction (PCR)

PCRBIO HS VeriFi Polymerase
PCRBIO VeriFi Polymerase
PCRBIO HS Taq DNA Polymerase
PCRBIO Taq DNA Polymerase
qPCRBIO SyGreen Mix
qPCRBIO SyGreen Blue Mix
qPCRBIO Probe Mix
qPCRBIO Probe Blue Mix
qPCRBIO cDNA Synthesis Kit

Our products

Product category: Polymerase chain reaction (PCR)
PCRBIO HS VeriFi Polymerase
PCRBIO HS VeriFi™ Polymerase is a versatile and robust proofreading enzyme with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

Features

AptaLock™ hot start technology for maximised sensitivity and specificity
Greater success with long and/or GC or AT-rich templates (17.5kb and over)
High temperature cycling – up to 100°C denaturation to better separate GC-rich sequences
100x higher fidelity than Taq DNA polymerase
Room temperature setup
Reaction mix stability for up to 24 hours both before and after PCR run
Generates blunt-end PCR products
Also available as a 2x ready mix with the option of a red dye for direct gel loading

Product category: Polymerase chain reaction (PCR)
PCRBIO VeriFi Polymerase
PCRBIO VeriFi™ Polymerase is a versatile and robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required. Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets.

Features

High temperature cycling – up to 100°C denaturation to better separate GC-rich templates
Increased PCR success rates with complex genomic templates (17.5kb and over)
High yields under standard and fast PCR conditions (10-30s/kb)
Efficient and specific amplification from challenging templates including GC and AT-rich sequences
100x higher fidelity than Taq DNA polymerase
Generates blunt-end PCR products
Available as a convenient 2x ready mix with the option of a red dye for direct gel loading

Product category: Polymerase chain reaction (PCR)
PCRBIO HS Taq DNA Polymerase
PCRBIO HS Taq DNA Polymerase is an advanced antibody-mediated hot start DNA polymerase designed for fast, highly specific and sensitive PCR. Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.

Features

Antibody-mediated hot start technology for unrivalled detection of low copy number templates
Increased PCR success rates with amplicons up to 6kb
Ultra-low background DNA
Advanced buffer chemistry including Mg and dNTP
Efficient specific amplification from GC and AT-rich sequences
High performance under both fast and standard cycling conditions
Gives increased PCR throughput with existing thermal cyclers
Stable at 25°C and 37°C for 4 weeks

Product category: Polymerase chain reaction (PCR)
PCRBIO Taq DNA Polymerase
PCRBIO Taq DNA Polymerase is an affordable, versatile and robust enzyme for all your everyday PCR applications including genotyping, screening and library construction. An enhanced 12-step purification strategy together with an optimised buffer system enable PCRBIO Taq DNA Polymerase to amplify with the highest speed, yield and specificity.

Features

Increased PCR success rates with amplicons up to 6kb
Ultra low background DNA
Advanced buffer chemistry including Mg and dNTPs
High yields under standard and fast PCR conditions
Efficient specific amplification from complex templates including GC and AT-rich sequences
Stable at 25°C and 37°C for 4 weeks

Product category: Polymerase chain reaction (PCR)
qPCRBIO SyGreen Mix
qPCRBIO SyGreen Mix combines a proprietary non-inhibiting intercalating dye with the latest advances in polymerase technology and buffer chemistry to give you fast, highly sensitive and reproducible real-time PCR. qPCRBIO SyGreen Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences, and is able to detect extremely low copy number targets with the highest efficiency.

Features

Rapid extension rate for early Ct values
Market-leading sensitivity – increased limit of detection
Specific amplification from complex templates including GC-rich sequences
Non-PCR inhibiting intercalating dye
Also available as an easy-to-see blue mix
Compatible with all standard and fast cycling real-time instruments

Product category: Polymerase chain reaction (PCR)
qPCRBIO SyGreen Blue Mix
This easy-to-see mix uses a non-reactive blue dye for easy sample visualisation during reaction setup, allowing fast, highly sensitive and reproducible real-time PCR with more precision and accuracy than ever. qPCRBIO SyGreen Blue Mix is particularly useful in reducing errors when loading small volume reactions or using white plates.

Features

Easy-to-see blue mix for greater pipetting precision and accuracy
Rapid extension rate for early Ct values
Market-leading sensitivity – increased limit of detection
Specific amplification from complex templates including GC-rich sequences
Non-PCR inhibiting intercalating dye
Compatible with all standard and fast cycling real-time instruments

Product category: Polymerase chain reaction (PCR)
qPCRBIO Probe Mix
Our universal probe kit is designed to give superior sensitivity and specificity in all probe-based real-time PCR assays, including TaqMan®, Scorpions® and molecular beacon probes. Reliably perform singleplex or multiplex expression studies and diagnostic assays with this robust, market-leading probe kit.

Features

High efficiency in multiplex reactions
Rapid extension rate for early Ct values
Market leading sensitivity – increased limit of detection
Efficient amplification from GC and AT-rich sequences
Antibody-mediated hot start technology
Compatible with all real-time PCR platforms
Also available as an easy-to-see blue mix

Product category: Polymerase chain reaction (PCR)
qPCRBIO Probe Blue Mix
qPCRBIO Probe Blue Mix is a universal probe kit that includes a non-reactive blue dye for easy sample visualisation and enhanced pipetting accuracy. Whether your application is for a singleplex or multiplex expression study or a diagnostic assay, qPCRBIO Probe Blue Mix is the robust choice for all your probe-based real-time PCR needs.

Features

Easy-to-see blue mix for greater pipetting precision
High efficiency in multiplex reactions
Rapid extension rate for early Ct values
Market leading sensitivity – increased limit of detection
Efficient amplification from GC and AT-rich sequences
Antibody-mediated hot start technology
Compatible on all real-time PCR platforms – standard and fast cycling conditions

Product category: Polymerase chain reaction (PCR)
qPCRBIO cDNA Synthesis Kit
High quality cDNA synthesis is essential for downstream real-time PCR analysis and successful expression studies. qPCRBIO cDNA Synthesis Kit is an easy-to-use 2 tube system specifically developed to generate cDNA for use in real-time PCR. The reverse transcriptase, buffer system and optimised blend of random hexamers with anchored oligo(dT) primers provide unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations.

Features

Unbiased representation of 5’ and 3’ mRNA transcript ends
Sensitive detection of low copy number transcripts
High cDNA yields from as little as 4pg total RNA
Reduced RNase H activity
Simple 2 tube system
5x buffer contains anchored oligo(dT), random hexamers, enhancers, dNTPs and MgCl2
20x thermostable reverse transcriptase blended with RNase inhibitor

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About Us

Company details
PCR Biosystems was founded in 2012 by two PCR experts who believed scientists deserved better performance and value from their PCR products. We wanted to do things differently.

We’ve developed a range of PCR reagents that maximise yield and sensitivity from the simplest to most challenging of reactions. On average, our products out­perform every competitor in terms of yield, specificity, sensitivity and speed. So your reactions have the best chance of working the way you want, first time.

Our portfolio covers a broad range of applications including real-time PCR, high fidelity PCR, hot start PCR, long PCR, PCR direct from crude samples and molecular diagnostic PCR. We offer a broad range of standard and custom solutions including bulk supply of reagents (both catalogue and custom), OEM manufacturing and expert technical support to help you achieve the most from our market-leading polymerases.

We’re a UK-based company with our manufacturing headquarters in London. The company’s quality management system meets the international standards of ISO 9001:2015 and ISO 13485:2016, meaning our products and processes achieve the highest possible standards for reliability, efficiency and quality. It might help explain why our customers say we’re one of their favourite companies to purchase PCR reagents from. To find out more, visit us in Hall 3, Stand E82.

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