Medical Products Supplies Companies

77, Heyford Park Upper Heyford, OX25 5HD Oxfordshire
United Kingdom of Great Britain and Northern Ireland
Telephone +44 (0) 1869 238033
Fax +44 (0) 1869 238034
info@2BScientific.com
http://www.2BScientific.com

Product Categories

Product categories

03 Diagnostic Tests
03.02 Immunochemistry testing, immunology testing
03.02.01 Immuno assay testing

Immuno assay testing

Our products

Product category: Immuno assay testing
Kits & Assays
Provided as fully-optimised, user-friendly formats, commercially-available kits and assays are an ideal solution to streamline research. By purchasing a product which contains a comprehensive protocol and all the necessary components to achieve reproducible, high-quality data, researchers can benefit from shortened experimental timelines. This approach frees up time which might otherwise be spent on assay development, optimisation and troubleshooting.

Product category: Immuno assay testing
ELISA
One of the most widely-used forms of off-the-shelf kit, enzyme-linked immunosorbent assays (ELISAs) are microplate-based immunoassays which rely on adsorption of a molecule to a solid surface. This solid surface could be the microplate itself, or alternatively a specialised bead may be used as the solid phase. Easy to perform, quantitative, and sensitive, ELISA has utility across every field of research. It also benefits from applicability to automation, meaning it is ideally-suited to high throughput screening campaigns.

Product category: Immuno assay testing
CLIA
Chemiluminescent immunoassay (CLIA) is a similar technique to ELISA, reliant on chemiluminescent detection. Chemiluminescence delivers increased sensitivity and a greater dynamic range than chromogenic detection, making CLIA ideal to detect low-abundance analytes.

The process of chemiluminescence involves conversion of a specialised substrate to product with the associated release of light energy which can be quantified. During CLIA, this reaction is typically catalysed by enzymes such as horseradish peroxidase (HRP) or alkaline phosphatase (AP), which are provided as antibody conjugates. CLIA detection requires a dedicated reader, capable of measuring a chemiluminescent readout.

Product category: Immuno assay testing
ELISpot
Enzyme-linked immunospot (ELISpot) assays are used to detect substances secreted by cells which have been cultured in vitro. To achieve this, the cells are grown on a surface which has been coated with a specific capture antibody. As the cells secrete biomolecules such as cytokines, these become bound to the immobilised antibody. Following removal of the cells, the secreted biomolecules can be visualised through direct or indirect detection in a method analogous to ELISA.

Product category: Immuno assay testing
FluoroSpot
Like an ELISpot assay, FluoroSpot is used to detect and analyse the secreted products of plated cells. However, instead of relying on a colorimetric readout, FluoroSpot uses fluorescently-labelled antibodies for detection. By measuring the intensity of the fluorescent readouts, the amount of each secreted product can be quantified.

Highly-sensitive and easy to perform, FluoroSpot can be used to analyse the secretion of multiple analytes from the same cell. It can also distinguish between different cell types in the same assay well and can inform the relative robustness of similar cell types within an assay by comparing spot sizes and fluorescent intensities. A further utility of FluoroSpot is to investigate the downstream effects of biomolecular co-secretion.

Analysis of FluoroSpot data requires a specialised reader, capable of measuring the volume of each spot and of detecting different wavelengths. Careful selection of fluorophores allows simultaneous analysis of multiple secreted molecules which may exhibit different kinetics, while fixed camera settings can eliminate user bias.

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About Us

Company details
With its headquarters in Oxfordshire, UK, 2BScientific is reinventing the supply of immunological reagents and kits to the European research market. At the heart of everything we do lies our mission statement: 2BScientific Ltd’s mission is to become the leading supplier of innovative life science reagents and services to the European research market.

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